Gene targeting using randomly inserted group II introns (targetrons) recovered from an Escherichia coli gene disruption library
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چکیده
منابع مشابه
Gene targeting using randomly inserted group II introns (targetrons) recovered from an Escherichia coli gene disruption library
The Lactococcus lactis Ll.LtrB group II intron retrohomes by reverse-splicing into one strand of a double-stranded DNA target site, while the intron-encoded protein cleaves the opposite strand and uses it to prime reverse transcription of the inserted intron RNA. The protein and intron RNA function in a ribonucleoprotein particle, with much of the DNA target sequence recognized by base-pairing ...
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Mobile group II introns ("targetrons") can be programmed for insertion into virtually any desired DNA target with high frequency and specificity. Here, we show that targetrons expressed via an m-toluic acid-inducible promoter from a broad-host-range vector containing an RK2 minireplicon can be used for efficient gene targeting in a variety of gram-negative bacteria, including Escherichia coli, ...
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26 Mobile group II introns (" targetrons ") can be programmed to insert into virtually any desired 27 DNA target with high frequency and specificity. Here, we show that targetrons expressed via an 28 m-toluic acid-inducible promoter from a broad host-range vector containing an RK2 mini-replicon 29 can be used for efficient gene targeting in a variety Gram-negative bacteria, including Escherichi...
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ژورنال
عنوان ژورنال: Nucleic Acids Research
سال: 2005
ISSN: 0305-1048,1362-4962
DOI: 10.1093/nar/gki649